科研项目及学术成果

胰腺外科科研实力强大，研究方向始终站在学科前沿。胰腺外科目前承担国家自然基金16项，十一五攻关子项目一项，卫生部临床重点专科项目一项，卫生部行业专项科研项目两项等部省级课题20余项，获得科研经费支持1500余万元。该中心在特殊类型胰腺炎独特病理生理机制、胰腺癌干细胞及分子生物学机制的深入研究，国家权威杂志发表科研论文500余篇，其中SCI收录78篇，被引用共1000余次，多项研究成果处于国内领先或达到国际先进水平。胰腺癌诊断评估与综合治疗抉择体系的临床与基础研究成果多次在国内外大型学术会议报告，并且在国内30余家大型综合性医院推广应用。举办国家级继续教育培训班，目前已培训学员3000余名。迄今为止，该中心已培养博士研究生50余名，硕士研究生100余名。同时也投入力量在胰腺移植、胰岛移植等方面作出了大胆尝试。鉴于该学科带头人王春友教授在该领域做出的杰出贡献，被授予美国外科医师协会会师，美国医师联盟外籍会员。

成果获奖：

1 胰腺癌诊断评估与综合治疗抉择体系的临床与基础研究 2012 湖北省科技进步奖 一等奖

2 胰腺癌诊断评估与综合治疗抉择体系的临床与基础研究 2012 武汉市科技进步奖 一等奖

3 降低重症急性胰腺炎死亡率的四个关键措施的推广应用 2010 湖北省科技成果推广奖 二等奖

4 重症急性胰腺炎的综合救治方案的系统化与细化研究 2005 教育部科技进步奖 二等奖

5 重症急性胰腺炎的综合救治方案的系统化与细化研究 2005 湖北省科技进步奖 一等奖

承担科研项目：

卫生部行业基金 胰腺癌早期诊断与综合治疗

卫生部行业基金 胰腺囊性肿瘤的诊断与规范化治疗

国家“十一五”科技支撑计划 胰腺癌综合治疗技术体系研究

卫生部重点学科研究基金 胰腺重症疾病诊疗技术规范建立

国家自然科学基金 胰腺癌干细胞分离、鉴定及其关键调控分子表达研究

国家自然科学基金 胰腺癌起源于胰腺导管细胞与骨髓间充质干细胞融合细胞的实验研究

国家自然科学基金 衰老调控因子SIRT1与K-ras对话在胰腺癌发生发展中的作用

国家自然科学基金 胰腺癌微环境内TLR4/9、HIF-1α表达及相互调控与肿瘤生物学行为关系的研究

国家自然科学基金 树突状细胞疫苗联合Foxp3 siRNA诱导抗胰腺癌干细胞免疫反应治疗胰腺癌的实验研究

国家自然科学基金 胰岛素通过PKB信号通路调控HIF-1α与胰腺癌局部浸润能力的关系 国家自然科学基金 低浓度二甲双胍选择性抑制胰腺癌干细胞增殖的机制及提高化疗疗效的实验研究

国家自然科学基金 细胞外微环境应力在肝细胞恶性转化中作用及其机制

国家自然科学基金 Toll样受体及其信号通路在肝缺血再灌注损伤中的作用

国家自然科学基金 H0320肝素对重症急性胰腺炎治疗作用的新认识——肝素干扰

国家自然科学基金 H1502聚ADP-核糖聚合酶1调控高迁移率族蛋白1细胞内定位的机制及意义研究

国家自然科学基金 H1511再认识高迁移率族蛋白1的促炎活性- - 高迁移率族蛋白1放大脂多糖炎症效应的细胞内、外机制研究

HMGB1/RAGE配受体结合的机制及意义研究发表SCI收录论文：

1. Huang L, Yu D, Wu C, Zhai K, Jiang G, Cao G, Wang C, Liu Y, Sun M, Li Z, Tan W, Lin D. Copy number variation at 6q13 functions as a long-range regulator and is associated with pancreatic cancer risk. Carcinogenesis. 2012; 33(1): 94-100.

2. Zhou W, Wang L, Gou SM, Wang TL, Zhang M, Liu T, Wang CY. ShRNA silencing glycogen synthase kinase-3 beta inhibits tumor growth and angiogenesis in pancreatic cancer. Cancer Lett. 2012;316(2):178-86.

3. Zhao G, Qin Q, Zhang J, Liu Y, Deng S, Liu L, Wang B, Tian K, Wang C. Hypermethylation of HIC1 Promoter and Aberrant Expression of HIC1/SIRT1 Might Contribute to the Carcinogenesis of Pancreatic Cancer. Ann Surg Oncol. 2012 May 3.

4. Gou S, Liu T, Li X, Cui J, Wan C, Wang C. Pancreatic ductal cells acquire mesenchymal characteristics through cell fusion with bone marrow-derived mesenchymal stem cells and SIRT1 attenuates the apoptosis of hybrid cells. Cells Tissues Organs. 2012;196(2):129-36.

5. Hu W, Li H, Wang C, Gou S, Fu L. Characterization of collagen fibers by means of texture analysis of second harmonic generation images using orientation-dependent gray level co-occurrence matrix method. J Biomed Opt. 2012 Feb;17(2):026007.

6. Hu W, Zhao G, Wang C, Zhang J, Fu L. Nonlinear optical microscopy for histology of fresh normal and cancerous pancreatic tissues. PLoS One. 2012;7(5):e37962. Epub 2012 May 24.

7. Fan P, Zhang JJ, Wang B, Wu HQ, Zhou SX, Wang CY, Zhang JH, Tian Y, Wu HS. Hypoxia-inducible factor-1 up-regulates the expression of Toll-like receptor 4 in pancreatic cancer cells under hypoxic conditions. Pancreatology. 2012;12(2):170-8.

8. Wu C, Miao XP, Huang LM, et al. Genome-wide association study identifies five loci associated with susceptibility to pancreatic cancer in Chinese populations. Nat Genet. 2011;44(1):62-6.

9. Yin T, Wei H, Leng Z, Yang Z, Gou S, Wu H, Zhao G, Hu X, Wang C. Bmi-1 Promotes the Chemoresistance, Invasion and Tumorigenesis of Pancreatic Cancer Cells. Chemotherapy. 2011; 57(6): 488-496.

10. Zhu S, Zhou Y, Wang L, Zhang J, Wu H, Xiong J, Zhang J, Tian Y, Wang C, Wu H. Transcriptional upregulation of MT2-MMP in response to hypoxia is promoted by HIF-1α in cancer cells. Mol Carcinog. 2011;50(10):770-80.

11. Wu HQ, Wang B, Zhu SK, Tian Y, Zhang JH, Wu HS. Effects of CPG ODN on biological behavior of PANC-1 and expression of TLR9 in pancreatic cancer. World J Gastroenterol. 2011; 17(8):996-1003.

12. Wei HJ, Yin T, Zhu Z, Shi PF, Tian Y, Wang CY. Expression of CD44, CD24 and ESA in pancreatic adenocarcinoma cell lines varies with local microenvironment. Hepatobiliary Pancreat Dis Int. 2011; 10(4):428-34.

13. Yin T, Wei H, Gou S, Shi P, Yang Z, Zhao G, Wang C. Cancer stem-like cells enriched in panc-1 spheres possess increased migration ability and resistance to gemcitabine. Int J Mol Sci. 2011; 12(3):1595-604.

14. Zhou W, Wang L, Gou SM, Wang TL, Zhang M, Liu T, Wang CY. ShRNA silencing glycogen synthase kinase-3 beta inhibits tumor growth and angiogenesis in pancreatic cancer. Cancer Letters. 2011, Nov 2.

15. Zhao G, Cui J, Zhang JG, Qin Q, Chen Q, Yin T, Deng SC, Liu Y, Liu L, Wang B, Tian K, Wang GB, Wang CY. SIRT1 RNAi knockdown induces apoptosis and senescence, inhibits invasion and enhances chemosensitivity in pancreatic cancer cells. Gene Ther. 2011; 18(9): 920-928.

16. Ling Y, Yang ZY, Yin T, Li L, Yuan WW, Wu HS, Wang CY. Heparin changes the conformation of high-mobility group protein 1 and decreases its affinity toward receptor for advanced glycation endproducts in vitro. Int Immunopharmacol. 2011;11(2):187-93.

17. Zhu S, Zhou Y, Wang L, Zhang J, Wu H, Xiong J, Zhang J, Tian Y, Wang C, Wu H. Transcriptional upregulation of MT2-MMP in response to hypoxia is promoted by HIF-1alpha in cancer cells. Mol Carcinog. 2011; 50(10): 770-780.

18. Li Y, Xu J, Zou H, Wang CY. 1-MT enhances potency of tumor cell lysate-pulsed dendritic cells against pancreatic adenocarcinoma by downregulating the percentage of Tregs. J Huazhong Univ Sci Technolog Med Sci. 2010; 30(3):344-348.

19. Gou S, Wang C, Liu T, Wu H, Xiong J, Zhou F, Zhao G. Spontaneous differentiation of murine bone marrow-derived mesenchymal stem cells into adipocytes without malignant transformation after long-term culture. Cells Tissues Organs. 2010; 191(3): 185-192.

20. Wang L, Zhou W, Wang CY, et al. Insulin promotes proliferative vitality and invasive capability of pancreatic cancer cells via hypoxia-inducible factor 1alpha pathway. J Huazhong Univ Sci Technolog Med Sci. 2010; 30(3):349-353.

21. Zhang JJ, Wu HS, Wang L, Tian Y, Zhang JH, Wu HL. Expression and significance of TLR4 and HIF-1alpha in pancreatic ductal adenocarcinoma. World J Gastroenterol. 2010;16(23):2881-8.

22. Cui J, Zhao G, Wang CY, et al. Antiproliferative effect of resveratrol in pancreatic cancer cells. Phytother Res. 2010; 24(11):1637-1644.

23. Zhao G, Cui J, Qin Q, et al. Mechanical stiffness of liver tissues in relation to integrin beta1 expression may influence the development of hepatic cirrhosis and hepatocellular carcinoma. J Sur Oncl. 2010; 102 (5): 482-489.

24. Gou S, Wang C, Liu T, Wu H, Xiong J, Zhou F, Zhao G. Spontaneous differentiation of murine bone marrow-derived mesenchymal stem cells into adipocytes without malignant transformation after long-term culture. Cells Tissues Organs. 2010;191(3):185-92.

25. Gang Z, Qi Q, Jing C, Wang C. Measuring microenvironment mechanical stress of rat liver during diethylnitrosamine induced hepatocarcinogenesis by atomic force microscope. Micro Res Tech. 2009; 72 (9): 672-678.

26. Zhang J, Wang H, Xiao Q, Liang H, Li Z, Jiang C, Wu H, Zheng Q. Hyaluronic acid fragments evoke Kupffer cells via TLR4 signaling pathway. Sci China C Life Sci. 2009;52(2):147-54.

27. Wang C, Liu T, Wu H, Xiong J, Zhou F, Tao J, Yang Z, Gou S. Duodenum-preserving total pancreatic head resection without segment resection of the duodenum for chronic pancreatitis. Langenbecks Arch Surg. 2009;394(3):563-8.

28. Xiong J, Zhu S, Zhou Y, Wu H, Wang C. Regulation of omega-3 fish oil emulsion on the SIRS during the initial stage of severe acute pancreatitis. J Huazhong Univ Sci Technolog Med Sci. 2009;29(1):35-8.

29. Hui W, Jinxiang Z, Heshui W, Zhuoya L, Qichang Z. Bone marrow and non-bone marrow TLR4 regulates hepatic ischemia/reperfusion injury. Biochem Biophys Res Commun. 2009;389(2):328-32.

30. Wang CY, Liu T, Wu HS, et al. Duodenum-preserving total pancreatic head resection without segment resection of the duodenum for chronic pancreatitis. Langenbecks Archives of Surgery. 2009; 394 (3): 563-568.

31. Zhou J, Wang CY, Liu T, Wu B, Zhou F, Xiong JX, Wu HS, Tao J, Zhao G, Yang M, Gou SM. Persistence of side population cells with high drug efflux capacity in pancreatic cancer. World J Gastroenterol. 2008;14(6):925-930.

32. Wang CY, Gou SM, Liu T, Wu HS, Xiong JX, Zhou F, Tao J. Differentiation of CD24- pancreatic ductal cell-derived cells into insulin-secreting cells. Dev Growth Differ. 2008; 50(8): 633-643.

33. Huang P, Wang CY, Gou SM, Wu HS, Liu T, Xiong JX. Isolation and biological analysis of tumor stem cells from pancreatic adenocarcinoma. World J Gastroenterol. 2008;14(24):3903-3907.

34. Wang CY, Wu H, Xiong J,et al. Pancreaticoduodenectomy with vascular resection for local advanced pancreatic head cancer: a single center retrospective study. J Gastrointest Surg. 2008; 12(12):2183-2190.

35. Zhou M, Zhang Q, Zeng Q, Qiu Y, Liu N, Zhu Y, Zhou T, Chen B, Wang C. Establishment of an infected necrotizing pancreatitis model by retrograde pancreatic duct injection of sodium taurocholate and E. coli in rats. J Huazhong Univ Sci Technolog Med Sci. 2008;28(1):73-6.

36. Yang ZY, Ling Y, Yin T, Tao J, Xiong JX, Wu HS, Wang CY. Delayed ethyl pyruvate therapy attenuates experimental severe acute pancreatitis via reduced serum high mobility group box 1 levels in rats.World J Gastroenterol. 2008;14(28):4546-50.

37. Wan CD, Wang CY, Liu T, Cheng R, Wang HB. Alleviation of ischemia/reperfusion injury in ob/ob mice by inhibiting UCP-2 expression in fatty liver. World J Gastroenterol. 2008;14(4):590-4.

38. Liu T, Wang CY, Gou SM, Wu HS, Xiong JX, Zhou J. PDX-1 expression and proliferation of duct epithelial cells after partial pancreatectomy in rats.World J Gastroenterol. 2008;14(6):925-30.

39. Li K, Tao J, Wang CY, et al. Effect of antisense oligodeoxynucleotide of vascular endothelial growth factor C on lymphangiogenesis and angiogenesis of pancreatic cancer. J Huazhong Univ Sci Technolog Med Sci. 2007;27(1):51-3.

40. Gou SM, Liu T, Wang CY, et al. Establishment of clonal colony-forming assay for propagation of pancreatic cancer cells with stem cell properties. Pancreas. 2007;34(4):429-435.

41. Zhou J, Wang CY, Liu T, Wu B, Zhou F, Xiong JX, Wu HS, Tao J, Zhao G, Yang M, Gou SM.Persistence of side population cells with high drug efflux capacity in pancreatic cancer. Hepatobiliary Pancreat Dis Int. 2007;6(4):424-9.

42. Liu T, Gou SM, Wang CY, et al. Pancreas duodenal homeobox-1 expression and significance in pancreatic cancer. World J Gastroenterol. 2007;13(18):2615-2618.

43. Yin T, Wang CY, Liu T, et al. Expression of snail in pancreatic cancer promotes metastasis and chemoresistance. J Surg Res. 2007;141(2):196-203.

44. Hu WG, Liu T, Xiong JX, Wang CY. Blockade of sonic hedgehog signal pathway enhances antiproliferative effect of EGFR inhibitor in pancreatic cancer cells. Acta Pharmacol Sin. 2007;28(8):1224-1230.

45. Zhang W, Long Y, Zhang J, Wang CY. Modulatory effects of EPA and DHA on proliferation and apoptosis of pancreatic cancer cells. J Huazhong Univ Sci Technolog Med Sci. 2007; 27(5):547-50.

46. Liu T, Wang CY, Yu F, et al. In vitro pancreas duodenal homeobox-1 enhances the differentiation of pancreatic ductal epithelial cells into insulin-producing cells. World J Gastroenterol. 2007; 13(39):5232-5237.

47. Zhang W, Wang C, Yang M, Wan C. Relationship between the apoptosis and notch-1 expression in acute pancreatitis. J Huazhong Univ Sci Technolog Med Sci. 2007;27(1):48-50.

48. Chen Q, Wang C, Jiang C, Chen D. Exogenous PTEN gene induces apoptosis in breast carcinoma cell line MDA468. J Huazhong Univ Sci Technolog Med Sci. 2007;27(1):61-4.

49. Jin X, Wang L, Wu HS, Zhang L, Wang CY, Tian Y, Zhang JH. N-acetylcysteine inhibits activation of toll-like receptor 2 and 4 gene expression in the liver and lung after partial hepatic ischemia-reperfusion injury in mice. Hepatobiliary Pancreat Dis Int. 2007;6(3):284-9.

50. Wang W, Yang S, Su Y, Xiao Z, Wang C, Li X, Lin L, Fenton BM, Paoni SF, Ding I, Keng P, Okunieff P, Zhang L. Enhanced antitumor effect of combined triptolide and ionizing radiation. Clin Cancer Res. 2007;13(16):4891-9.

51. Wan C, Wang C, Liu T, Wang H, Yang Z. Experimental study on the cryopreservation of LLC-PK1 epithelial cells with hypoxic UW solution. J Huazhong Univ Sci Technolog Med Sci. 2007;27(4):426-8.

52. Wan C, Wang C, Liu T, Cheng R, Yang Z. Preparation and preservation of hypoxia UW solution. J Huazhong Univ Sci Technolog Med Sci. 2007;27(5):554-6.

53. Lü P, Liu F, Yan L, Peng T, Liu T, Yao Z, Wang CY. Stem cells therapy for type 1 diabetes. Diabetes Res Clin Pract. 2007;78(1):1-7.

54. Li K, Tao J, Li T, Yu Z, Yang Z, Wu H, Xiong J, Wang C. Effect of antisense oligodeoxynucleotide of vascular endothelial growth factor C on lymphangiogenesis and angiogenesis of pancreatic cancer. J Huazhong Univ Sci Technolog Med Sci. 2007;27(1):51-3.

55. Wei HY, Wang CY, Chen L, et al. Proliferating cell nuclear antigen, survivin, and CD34 expressions in pancreatic cancer and their correlation with hypoxia-inducible factor 1alpha. Pancreas. 2006;32(2):159-163.

56. Chen Q, Wang C, Chen D, Chen J, Jiang C, Zheng H. Construction and expression of human PTEN tumor suppressor gene recombinant adenovirus vector. J Huazhong Univ Sci Technolog Med Sci. 2006;26(3):325-8.

57. Wang L, Xu JB, Wu HS, Zhang JX, Zhang JH, Tian Y, Wang CY. The relationship between activation of TLR4 and partial hepatic ischemia/reperfusion injury in mice.Hepatobiliary Pancreat Dis Int. 2006;5(1):101-4

58. Xiong J, Zhu ZH, Liu JS, Wang Y, Wu HS. The expression of toll-like receptor 2, 4 of livers in mice with systemic inflammatory response syndrome.Hepatobiliary Pancreat Dis Int. 2006;5(1):143-6.

59. Zhang L, Wu HS, Chen Y, Guo XJ, Wang L, Wang CY, Zhang JH, Tian Y. Role of nitric oxide in Toll-like receptor 2 and 4 mRNA expression in liver of acute hemorrhagic necrotizing pancreatitis rats. World J Gastroenterol. 2006;12(3):485-8.

60. Zhang L, Wu HS, Chen Y, Guo XJ, Wang L, Wang CY, Zhang JH, Tian Y. Role of nitric oxide in Toll-like receptor 2 and 4 mRNA expression in liver of acute hemorrhagic necrotizing pancreatitis rats. World J Gastroenterol. 2006;12(3):485-8.

61. Wang H, Zhang J, Wu H, Jiang C, Zheng Q, Li Z. Inhibition of RAW264.7 macrophage inflammatory cytokines release by small haparin RNAi targeting TLR4. J Huazhong Univ Sci Technolog Med Sci. 2006;26(5):500-3.

62. Tao J, Xiong J, Li T, Yang Z, Li X, Li K, Wu H, Wang C. Correlation between protein expression of PTEN in human pancreatic cancer and the proliferation, infiltration, metastasis and prognosis. J Huazhong Univ Sci Technolog Med Sci. 2006;26(4):444-7.

63. Tao J, Li T, Li K, Xiong J, Yang Z, Wu H, Wang C. Effect of HIF-1alpha on VEGF-C induced lymphangiogenesis and lymph nodes metastases of pancreatic cancer. J Huazhong Univ Sci Technolog Med Sci. 2006;26(5):562-4.

64. Liu T, Wang C, Wan C, Xiong J, Zhou F. Proliferation and differentiation of duct epithelial cells after partial pancreatectomy in rats. J Huazhong Univ Sci Technolog Med Sci. 2006;26(5):567-9.

65. Yin T, Wang C, Liu T, Zhao G, Zhou F. Implication of EMT induced by TGF-beta1 in pancreatic cancer. J Huazhong Univ Sci Technolog Med Sci. 2006;26(6):700-2.

66. Yang M, Liu W, Wang CY, et al. Proteomic analysis of differential protein expression in early process of pancreatic regeneration in pancreatectomized rats. ACTA Pharmacologica Sinica. 2006; 27 (5): 568-578.

67. Wang W, Wang CY, Dong JH, Chen X, Zhang M, Zhao G. Identification of effective siRNA against K-ras in human pancreatic cancer cell line MiaPaCa-2 by siRNA expression cassette. World J Gastroenterol. 2005;11(13):2026-31.

68. Wu HS, Zhang L, Chen Y, Guo XJ, Wang L, Xu JB, Wang CY, Zhang JH. Effect of nitric oxide on toll-like receptor 2 and 4 gene expression in rats with acute lung injury complicated by acute hemorrhage necrotizing pancreatitis. Hepatobiliary Pancreat Dis Int. 2005;4(4):609-13.

69. Lü P, Liu F, Wang CY, Chen DD, Yao Z, Tian Y, Zhang JH, Wu YH. Gender differences in hepatic ischemic reperfusion injury in rats are associated with endothelial cell nitric oxide synthase-derived nitric oxide. World J Gastroenterol. 2005;11(22):3441-5.

70. Lu P, Liu F, Yao Z, Wang CY, Chen DD, Tian Y, Zhang JH, Wu YH. Nitrite-derived nitric oxide by xanthine oxidoreductase protects the liver against ischemia-reperfusion injury. Hepatobiliary Pancreat Dis Int. 2005;4(3):350-5.

71. Zhou F, Wang C, Xiong J, Wan C, Zheng C. Experience in diagnosis and treatment of bleeding complications in severe acute pancreatitis by TAE. J Huazhong Univ Sci Technolog Med Sci. 2005;25(2):182-4.

72. Zhang JX, Wu HS, Wang H, Zhang JH, Wang Y, Zheng QC. Protection against hepatic ischemia/reperfusion injury via downregulation of toll-like receptor 2 expression by inhibition of Kupffer cell function. World J Gastroenterol. 2005;11(28):4423-6.

73. Zhang J, Wu H, Wang L, Zhang J, Wang H, Zheng Q. TLR2 mRNA upregulation in ischemic lobes in mouse partial hepatic ischemia/reperfusion injury model. J Huazhong Univ Sci Technolog Med Sci. 2004;24(2):144-6.

74. Yang Z, Wang C, Tao J, Xiong J, Wan C, Zhou F. Effect of early hemofiltration on pro- and anti-inflammatory responses and multiple organ failure in severe acute pancreatitis. J Huazhong Univ Sci Technolog Med Sci. 2004;24(5):456-9.

75. Liu T, Wang C, Wan C, Xiong J, Xu Y, Zhou F. PDX-1 expression in pancreatic ductal cells after partial pancreatectomy in adult rats. J Huazhong Univ Sci Technolog Med Sci. 2004;24(5):464-6.

76. Wu HS, Zhang JX, Wang L, Tian Y, Wang H, Rotstein O. Toll-like receptor 4 involvement in hepatic ischemia/reperfusion injury in mice. Hepatobiliary Pancreat Dis Int. 2004;3(2):250-3.

77. Tao J, Wang C, Chen L, et al. Diagnosis and management of severe acute pancreatitis complicated with abdominal compartment syndrome. J Huazhong Univ Sci Technolog Med Sci. 2003;23(4):399-402.

78. Zhao G, Wang CY, Wang F, Xiong JX. Clinical study on nutrition support in patients with severe acute pancreatitis. World J Gastroenterol. 2003;9(9):2105-8.